Fig. 4.

Recruited cells expressed increased mRNA levels that are attenuated by axitinib. RNA was extracted from BAL cells isolated from lungs of control mice and mice with Tsc2-null lesions treated with diluent or axitinib. Expression of gene markers was quantified by quantitative RT-PCR, as described in materials and methods. Ct values obtained were normalized to β-actin signals and further analyzed using the relative quantization (ΔΔCt) method. Values are fold change (means ± SE, n = 3–5 per each group). Comparisons between groups were made by two-way ANOVA and Bonferroni correction for multiple comparisons. P < 0.05 vs. *control/diluent group and #Tsc2-null/diluent group.