Fig. 1.

Histone deacetylase (HDAC) inhibition protects against the lipopolysaccharide (LPS)-mediated decrease in transendothelial resistance (TER). Human lung microvascular endothelial cells (HLMVEC) were grown to confluence on ECIS arrays (8W10E+). Once a constant resistance was attained, the cells were treated with either vehicle (0.1% DMSO) or 1 μM panobinostat (Pan; A) or 2 μM trichostatin (TSA; B) for 2 h. LPS [1 endotoxin unit (EU)/ml] was then added as indicated, and TER values were measured using ECIS Zθ. Normalized resistance was calculated and plotted as a function of time. P < 0.01 from vehicle (*) and LPS (#); n ≥ 4 experiments.