Fig. 6.

Combined inhibition of HDAC3 and -6 prevents MLC phosphorylation and abrogates Hsp90 chaperone function. A: HLMVEC were grown in 6-well plates. Confluent monolayers were treated with vehicle, LPS, 5 μM RGFP-966 + 5 μM tubastatin (RT), or RT for 2 h followed by exposure to LPS (1 EU/ml) for 2 h. Cells were then lysed and immunoblotted with antibodies against ppMLC2 and MLC2. P < 0.05 from vehicle (*) and LPS (#); n = 4. B: cells were treated as in A and immunoblotted with antibodies against pAkt and Akt. P < 0.05 from vehicle (*) and LPS (#); n = 4.