Fig. 7.

Small-interfering RNA (siRNA)-induced downregulation of both HDAC3 and HDAC6 protects against LPS-induced EBD. A and B: HLMVEC were transfected with either scrambled siRNA or siRNAs against both HDAC3 and -6. Later (48 h) transfected cells were lysed and immunoblotted with antibodies against HDAC3 (A) and HDAC6 (B). *P < 0.05 and ***P < 0.001; n = 4. C: HLMVEC were transfected with either scrambled siRNA or siRNAs against both HDAC3 and -6. Later (24 h) transfected cells were added to ECIS arrays (8W10E+) and allowed to grow to confluence. *P < 0.01 from scrambled siRNA; n = 4. D: the same cells and arrays as in C. Following 24 h exposure to siRNA, TER was normalized and is presented as time = 0. The cells were then treated with either vehicle (PBS) or LPS (1 EU/ml) as indicated, and TER values were monitored. P < 0.05 from vehicle (*) and LPS (#); n = 4.