Skip to main content
. 2015 Aug 31;126(25):2695–2703. doi: 10.1182/blood-2015-05-644104

Figure 2.

Figure 2

Generation of RIAM−/− mice. (A) Schematic representation of RIAM targeting. The RIAM locus of murine ES cells was targeted by homologous recombination to generate an allele with loxP sites flanking exons 3 and 4. The resulting RIAM conditional mice were crossed with a Cre-deleter strain to remove exons 3 and 4, along with the Neo cassette, and thereby generate RIAM+/− mice that were crossed to produce RIAM−/− mice. (B) Immunmoblot for RIAM and RhoGDI of lysates of T and B cells isolated from spleens of RIAM+/+ and RIAM−/− mice. (C) Immunoblot for lamellipodin and RhoGDI of lysates of of MDA-MB-231 human breast cancer cells, as well as the extracts of brain, spleen, and platelets from mice with the indicated genotype.