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. 2015 Aug 31;126(25):2695–2703. doi: 10.1182/blood-2015-05-644104

Figure 4.

Figure 4

RIAM−/− lymphocytes are defective in integrin-dependent adhesion and migration. (A-B) Expression of LFA-1 (αL; A) and VLA-4 (α4; B) in spleen-derived T and B cells from RIAM+/+ or RIAM−/− mice was determined by flow cytometry. (C-D) Percentage of T (left) or B (right) cells (resting or stimulated as indicated) from spleens of RIAM+/+ or RIAM−/− mice adherent to ICAM-1 (C) or VCAM-1 (D) coated wells after washing. (E) T-cell adhesion to ICAM-1 ±SDF-1 measured in a flow chamber. (F) B-cell migration measured as percentage of cells that cross the membrane of a modified Boyden chamber toward media ± SDF-1. Plots show mean ± SD; n = 4 (C-D) or n = 3 (E-F). *P < .05, **P < .01, ***P < .001.