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. 2015 Dec 18;5:18191. doi: 10.1038/srep18191

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Copyright © 2015, Macmillan Publishers Limited

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Relative deamination activity of full-length A3G (A) as a function of increasing zinc concentrations, and of A3A (B) at increasing stoichiometric ratios of zinc. A representative gel showing the resolution of non-deaminated “ND” and deaminated “D” substrate of A3G is shown in (A). (C) Relative deamination activity of A3G^CTD w.t. (striped bars) and H248S/H250S mutant (black bars) at increasing stoichiometric ratios of zinc. Data represent mean ± S.E.M. of three biological repeats. P-values (one-way ANOVA) calculated in comparison to control samples (absence of extra zinc). (D) ICP-AES data (mg/L) represent means ± S.E.M. of duplicate measurements at two concentrations (μM) of A3G^CTD w.t. and H248S/H250S (HH/SS) compared to ZnCl₂ as a control.