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. 2015 Dec 18;5:18191. doi: 10.1038/srep18191

Figure 4. A G56N mutation to the NTD of full-length A3G does not bestow deamination activity.

Figure 4

Relative deamination activity of full-length A3G w.t. and mutants: G56N, E259Q and the double mutant G56N/E259Q (GN/EQ). Error bars (S.E.M) represent three biological repeats. Protein concentrations were normalized prior to assessing deamination activity and standardization is demonstrated by α-myc of purified samples (α-myc normalized). α-myc on the lysate demonstrates significantly decreased expression of A3G G56N/E259Q mutant on a comparable α-actin background. Sequence alignment of loop-7 from A3GCTD, A3GNTD and A3FCTD is shown.