Fig. 4.
Deletion in S10 may decrease the binding affinity of TGC by remodeling the ribosome. The position of the S10 mutations (S10R53Q-Δ54-57ATHK) within the context of the ribosome suggests that it may indirectly alter TGC binding. The costructure of the Thermus thermophiles 70S ribosome with TGC (yellow sticks) is shown (PDB 4G5T). For clarity, only S10 and the structures proximal to the TGC-binding pocket are shown. The mutated residues are located at the tip of a loop (residues 53–61) and are highlighted by red spheres at the carbon alpha position. The S10 loop does not directly contact TGC but instead interacts with several portions of the 16S rRNA (gray) that comprise the TGC-binding pocket (Jenner et al. 2013).