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. 2015 Dec 18;5:18327. doi: 10.1038/srep18327

Figure 1. PDLIM1 is a cytoplasmic protein expressed in dendritic cells.

Figure 1

(a) Western blot analysis of PDLIM1 and PDLIM4 expression in primary immune cells. Whole cell lysates were subjected to immunoblot (IB) with anti-PDLIM1, PDLIM4 and HSP90 antibodies. Western blots are representative of at least three independent experiments. (b) Real-time RT-PCR analysis of PDLIM3 expression in mouse tissues and primary immune cells. Data are means ± SD from at least three independent experiments. (c) Bone-marrow derived dendritic cells (BMDC) and mouse embryonic fibroblasts (MEF) were fractionated into cytoplasmic (C) and nuclear (N) fractions, which were then analyzed by Western blotting with anti-PDLIM1 antibody. The purity of the fractionations was confirmed by anti-cdc37 (cytoplasm) or anti-LSD1 (nucleus) antibody. Western blots are representative of at least three independent experiments. (d) Cytoplasmic localization of PDLIM1 in MEF was determined by indirect immunofluorescence with an in situ proximity-ligation assay. DAPI, nuclear staining. Original magnification, x630. Data are representative of at least three independent experiments.

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