Fig. 2.

Curcumin inhibits activation of a non-selective cation current in hepatocytes treated with paracetamol or H₂O₂. (A) Averaged current–voltage plots recorded in response to 100 ms voltage ramps between −120 and 120 mV in untreated hepatocytes (control, n=5), hepatocytes treated with 10 mM paracetamol for 60 min in control bath solution (paracetamol, n=5), and hepatocytes treated with 10 mM paracetamol and 5 µM curcumin for 60 min (paracetamol+curcumin, n=6). (B) The average amplitude of TRPM2-mediated Na⁺ current, obtained by replacing Na⁺ in the bath solution with NMDG⁺, in untreated hepatocytes (control) and hepatocytes treated with 10 mM paracetamol in the presence or absence of 5 µM curcumin for 60 min. (C) Averaged current–voltage plots recorded in response to 100 ms voltage ramps between −120 and 120 mV in untreated hepatocytes (control, n=5), hepatocytes treated with 0.5 mM H₂O₂ for 30 min (H₂O₂, n=5), and hepatocytes treated with 0.5 mM H₂O₂ in the presence of 5 µM curcumin for 30 min (H₂O₂+curcumin, n=5). (D) The average amplitude of TRPM2-mediated Na⁺ current, obtained by replacing NaCl in the bath solution with NMDGCl, in untreated hepatocytes (control) and hepatocytes treated with 0.5 mM H₂O₂ for 30 min, in the presence or absence of 5 µM curcumin. Standard errors in panels A and C are omitted for clarity.