Figure 6. Analysis of cell motility and viability via time-lapse imaging of w-SCOPE.

(a) The initial frame of the time-lapse images of the HEK-293 cell line, representing start time of Paclitaxel treatment, is labelled with red, purple and white points, as well as red circles indicating cells that move or disappear respectively. (b) The final frame of the HEK-293 trial, representing the 12 hours after Paclitaxel treatment, is shown. White colored trails indicate the paths of cells that migrated during the time-lapse period. Red trails indicate the paths of cells that migrated and eventually died before the end of the period. Purple colored trails indicate the merging of two or more cells. Red circles indicate the former location of cells that died without obvious moving. (c) to (d) The initial frame and final frame of the time-lapse data for the MCF-7 cell line treated with paclitaxel are similarly labelled and analyzed. When comparing (b) and (d), it is shown that MCF-7 cell line displays relatively higher mortality compared to motility following exposure to paclitaxel, and the HEK-293 line displayed comparatively higher motility and tendency of aggregation.