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. 2015 Dec 18;5:18412. doi: 10.1038/srep18412

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(a) Accumulation of TMV and its mutants positive and negative strand viral RNAs in transfected Nicotiana benthamiana protoplasts at 24, 48 and 72 hpt, respectively. Figure 2a represents the results of qRT-PCR. (b) RNA accumulation level and (c) coat protein expression of TMV and its mutants as determined by a regular RT-PCR and Western blot in transfected N. benthamiana protoplasts at 24, 48 and 72 hpt, respectively. The CP gene accumulation of TMV(62A+UPD) was reduced over time and its CP expression was not detected.