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. 2015 Dec 18;5:18412. doi: 10.1038/srep18412

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TMV sequence starts from 6178 nt to the 3′-end (6395 nt), consisting of 11 nucleotides and UGA stop codon of TMV-CP gene, UPD and TLS. The mutants were added with 24 nt, 42 nt and 62 nt internal poly(A) tracts, respectively, downstream of the CP gene UGA stop codon.