Fig. 1.

Effects of chronic EtOH on hepatic lipid accumulation and fibrosis in SV and GSTA4^−/− mice. Paraffin embedded formalin fixed tissue sections were analyzed immunohistochemically using polyclonal antibodies directed against ADPH (Panels A–D) or stained with Sirius red (Panels E–M). Top panels (A–D); adipophilin staining of tissue sections (Original magnification 400X). Middle panels (E–H); Picrosirius red staining of liver sections (original magnification 200X). Bottom panels (I–M); Polarized light exposure of picrosirius red staining. Figures are representative of three PF/EtOH groups of SV and GSTA4^−/− mice respectively.