Table 1.
Total number of proteins identified to be carbonylated in each fraction. 500 μg of cytosolic (cyto), mitochondrial (mito) and microsomal (micro) extracts were treated with biotin hydrazide and subjected to LC-MS/MS analysis as described in methods.
| Table 1 | Fraction | Number of animals used | # Identified proteins |
|---|---|---|---|
| All fractions (PF/EtOH) | All | 20 | 829 |
| SV PF | mito | 5 | 214 |
| SV EtOH | mito | 5 | 230 |
| GSTA4−/− PF | mito | 5 | 389 (+1.81-fold) |
| GSTA4−/− EtOH | mito | 5 | 371 (+1.61-fold) |
| SV PF | cyto | 5 | 238 |
| SV EtOH | cyto | 5 | 244 |
| GSTA4−/− PF | cyto | 5 | 224 (−0.06-fold) |
| GSTA4−/− EtOH | cyto | 5 | 195 (−0.13-fold)) |
| SV PF | micro | 4 | 209 |
| SV EtOH | micro | 4 | 191 |
| GSTA4−/− PF | micro | 3 | 116 (−0.45-fold) |
| GSTA4−/− EtOH | micro | 3 | 133(−0.31-fold) |