Figure 4. Effect of phosphofructokinase knockdown on cell shape.

Two types of phosphofructokinases (PFKs, PFK-M and PFK-L) are expressed in undifferentiated and differentiated podocytes (A). Transduction with adenovirus carrying shRNA for either PFK-M or PFK-L reduced their respective expression (B,C). Reduced PFK-L, but not PFK-M, expression significantly reduced intracellular L-lactate concentration (D). Phalloidin-labeled actin, showing that blocking glycolysis with PFK-L knockdown impaired lamellipodia formation (E), and quantification (F). Bar, 50 μm. Cropped gels/blots are shown in (A–C). Each blot was run under the same experimental conditions. Size markers indicate the cropped level in each figure. Full length gels and blots for each figure are shown in Supplemental Figure 2.