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. 2015 Dec 18;5:18575. doi: 10.1038/srep18575

Figure 7. Roles of OXPHOS and glycolysis in differentiating podocytes.

Figure 7

Antimycin reduced intracellular ATP levels 31% in undifferentiated cells and 24% in differentiating podocytes, but 49% in differentiated podocytes (A). In contrast, blocking glycolysis with 2-DG and pyruvate reduced ATP levels by 88% in undifferentiated cells, 86% in differentiating podocytes and 56% in differentiated podocytes (B). 2-DG with pyruvate inhibited lamellipodia formation, whereas antimycin had a minor effect (C). White arrows indicate lamellipodia formation whereas dotted arrows show a lack of lamellipodia formation in C. Blocking glycolysis significantly increased the number of TUNEL positive differentiating podocytes, whereas antimycin had no effect (D). sh-PFKL247 and sh-PFKL-2428 significantly reduced synaptopodin expression in differentiating podocytes in nonpermissive conditions (E). Antimycin also reduced synaptopodin mRNA expression in differentiating podocytes (F). Bar, 50 μm.