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(a) Heated wild type (7 μM) and mutant BAP1 solution was ultracentrifuged and the pellet containing aggregates of BAP1 was subjected to dot blot assay by anti-A11 and anti-OC antibodies. (b) Aggregates of wild type and mutant BAP1were added to HEK293T cells and cell viability was measured by MTT reduction assay after 24 h. Data represents results of four independent experiments.
