FIG. 3.

IL-8, TNF-α, and βD4 gene expression following suIFN-α treatments. IL-8, TNF-α, and βD4 gene expression was investigated in control untreated, suIFN-α, and mock plasmid-treated IPEC-J2 cells. Data are normalized on the values of mock plasmid-treated cells and expressed as ΔΔCt, where ΔΔCt = (ΔCt observed in mock-treated cells) − (ΔCt observed in IFN-treated cells or untreated cells). The asterisks indicate significant differences between values obtained from mock- and IFN-treated cells. *P < 0.05, **P < 0.01, and ***P < 0.001. TNF-α, tumor necrosis factor α.