FIG. 4.

IL-1β, IFN-β, and NF-kB gene expression following IFN-α treatments. IL-1β, IFN-β, and NF-kB gene expression was investigated in control untreated, suIFN-α, and mock plasmid-treated IPEC-J2 cells. Data are normalized on the values of control, untreated cells, and expressed as ΔΔCt, where ΔΔCt = (ΔCt observed in untreated cells) − (ΔCt observed in IFN- or mock-treated cells). The asterisks indicate significant differences between values obtained from untreated and IFN-treated cells. *P value < 0.05, **P < 0.01, and ***P < 0.001. T indicates a tendency to significance (P < 0.1). NF-kB, nuclear factor-κB.