Table 1.

Sequences of the Primers Used in Real-Time PCR

Gene	Protein	Primers	Accession No. NCBI
B2M	β2-Microglobulin	F:5′-CGCCCCAGATTGAAATTGATTTGC-3′	NM_213978.1
		R:5′-GCTATACTGATCCACAGCGTTAGG-3′
IL-1β	Interleukin-1β	F:5′-AATTCGAGTCTGCCCTGTACCC-3′	NM_001005149
		R:5′-GCCAAGATATAACCGACTTCACCA-3′
IL-4	Interleukin-4	F:5′-GGACACAAGTGCGACATCA-3′	NM_214123.1
		R:5′-GCACGTGTGGTGTCTGTA-3′
IL-6	Interleukin-6	F:5′-TGGCTACTGCCTTCCCTACC-3′	NM_214399
		R:5′-CAGAGATTTTGCCGAGGATG-3′
IL-8	Interleukin-8	F:5′-TTCGATGCCAGTGCATAAATA-3′	M86923
		R:5′-CTGTACAACCTTCTGCACCCA-3′
IL-10	Interleukin-10	F:5′-AGCCAGCATTAAGTCTGAGAA-3′	HQ236499.1
		R:5′-CCTCTCTTGGAGCTTGCTAA-3′
TNF-α	TNF-α	F:5′-TGCCTACTGCACTTCGAGGTTATC-3′	NM_214022
		R:5′-GTGGGCGACGGGCTTATCTG-3′
IFN-β	Interferon-β	F:5′-AGTTGCCTGGGACTCCTCAA-3′	NM_21455
		R:5′-CCTCAGGGACCTCAAAGTTCAT-3′
βD-1	β-Defensin-1	F:5′-CTGTTAGCTGCTTAAGGAATAAAGGC-3′	NM_213838
		R:5′-TGCCACAGGTGCCGATCT-3′
βD-2	β-Defensin-2	F:5′-CCAGAGGTCCGACCACTACA-3′	NM_214442
		R:5′-GGTCCCTTCAATCCTGTTGAA-3′
βD-3	β-Defensin-3	F:5′-CTTCCTATCCAGTCTCAGTGTTCTGC-3′	AY460575.1
		R:5′-GGCTTCTGTAGACTTCAAGGAGACAT-3′
βD-4	β-Defensin-4	F:5′-GTGGCTTGGATTTGAGGAGAGAGT-3′	AY460576.1
		R:5′-AGTGATACACAGGCCTGGAAGGAT-3′
IL-18	Interleukin-18	F:5′-CGTGTTTGAGGATATGCCTGATT-3′	AF191088.1
		R:5′-TGGTTACTGCCAGACCTCTAGTGA-3′
SOCS1	SOCS1	F:5′-TTCTTCGCCCTCAGTGTGAA-3′	HM462248.1
		R:5′-GGCCTGGAAGTGCACGC-3′
TLR-4	Toll-like receptor	F:5′-TGGCAGTTTCTGAGGAGTCATG-3′	AB188301.2
		R:5′-CCGCAGCAGGGACTTCTC-3′
NF-kB1	Nuclear factor κB	F:5′-CCCATGTAGACAGCACCACCTATGAT-3′	NM_001048232
		F:5′-ACAGAGGCTCAAAGTTCTCCACCA-3′

Supernatants of CHO cells were collected at 24 and 48 h after transfection with mock and suIFN-α plasmids. IFN-α titers were measured in MDBK cells by a cpe inhibition assay with VSV.

CHO, Chinese hamster ovary; cpe, cytopathic effect; IFN, interferon; MDBK, Madin–Darby bovine kidney; PCR, polymerase chain reaction; SOCS1, suppressor of cytokine signaling 1; TNF, tumor necrosis factor; VSV, vesicular stomatitis New Jersey virus.