Table 3.
Gene Expression in Terms of Ct Values in IPEC-J2 Cells After Treatments with Porcine suIFNs-α and rIFN-α1
| Cytokine expression | |||||
|---|---|---|---|---|---|
| Treatment | IL-4 | IL-6 | IL-18 | SOCS1 | bD3 |
| rIFN-α1a | 20.0 ± 0.0 | 6.7 ± 0.4 | 7.6 ± 0.1 | 17.4 ± 4.6 | 15.2 ± 0.7 |
| IFN-α2 | 20.0 ± 0.0 | 6.1 ± 0.9 | 9.7 ± 0.2 | 15.1 ± 4.3 | 17.0 ± 0.7 |
| IFN-α3 | 20.0 ± 0.0 | 4.6 ± 0.2 | 8.1 ± 0.4 | 15.5 ± 0.1 | 13.6 ± 0.7 |
| IFN-α4 | 20.0 ± 0.0 | 4.5 ± 0.4 | 7.6 ± 0.4 | 12.5 ± 0.1 | 14.4 ± 0.3 |
| IFN-α6 | 18.5 ± 2.6 | 6.2 ± 0.2 | 6.3 ± 0.1 | 18.8 ± 0.1 | 14.6 ± 0.7 |
| IFN-α7 | 18.8 ± 2.0 | 6.7 ± 0.1 | 6.0 ± 0.2 | 13.1 ± 0.1 | 15.3 ± 0.5 |
| IFN-α8 | 20.0 ± 0.0 | 6.7 ± 0.0 | 6.3 ± 0.1 | 13.1 ± 0.2 | 15.2 ± 0.6 |
| IFN-α9 | 20.0 ± 0.0 | 5.9 ± 0.7 | 7.2 ± 0.4 | 17.2 ± 5.0 | 12.9 ± 0.8 |
| IFN-α10 | 20.0 ± 0.0 | 5.2 ± 0.1 | 6.3 ± 0.0 | 10.8 ± 0.1 | 15.6 ± 3.8 |
| IFN-α11 | 20.0 ± 0.0 | 5.4 ± 0.1 | 6.0 ± 0.2 | 9.6 ± 0.9 | 17.7 ± 4.0 |
| IFN-α12 | 20.0 ± 0.0 | 4.8 ± 0.3 | 7.4 ± 0.1 | 11.8 ± 0.1 | 14.9 ± 1.0 |
| IFN-α13 | 20.0 ± 0.0 | 4.9 ± 0.2 | 7.8 ± 0.3 | 12.5 ± 1.6 | 15.3 ± 0.6 |
| IFN-α14 | 20.0 ± 0.0 | 6.0 ± 0.7 | 7.4 ± 0.2 | 11.9 ± 0.5 | 15.2 ± 0.3 |
| IFN-α15 | 20.0 ± 0.0 | 5.4 ± 0.3 | 6.8 ± 0.1 | 11.5 ± 0.3 | 14.6 ± 0.2 |
| IFN-α16 | 20.0 ± 0.0 | 5.3 ± 0.1 | 7.0 ± 0.1 | 11.4 ± 0.1 | 14.8 ± 0.6 |
| IFN-α17 | 20.0 ± 0.0 | 5.1 ± 0.2 | 7.4 ± 0.3 | 11.3 ± 0.1 | 16.4 ± 1.3 |
| Mock | 20.0 ± 0.0 | 5.5 ± 0.4 | 7.4 ± 1.9 | 11.7 ± 1.0 | 14.7 ± 1.8 |
| Untreated cells | 20.0 ± 0.0 | 5.3 ± 0.9 | 7.2 ± 7.4 | 12.2 ± 2.4 | 14.8 ± 1.9 |
Data are expressed as mean Ct ± 1 standard deviation.
a
Recombinant protein purchased from PBL Biomedical Laboratories.