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. 2015 Dec 1;35(12):969–980. doi: 10.1089/jir.2014.0235

FIG. 3.

FIG. 3.

The mRNA expression of TGF-β1 in CD4+CD25+ Tregs from BALB/c mice. (A) In vivo, TGF-β1 mRNA expression shown by reverse transcription–polymerase chain reaction (RT-PCR) analysis in CD4+CD25+ Tregs was significantly upregulated after polymicrobial sepsis and it could be downregulated using PC61. (B) In vitro, mRNA level of TGF-β1 in CLP-24-h or CLP-48-h group was markedly increased. Expression of β-actin served as an internal control. Statistical significance: *P<0.01 as CLP group versus sham group; #P<0.01 as PC61 group versus CLP group.