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. 2015 Nov 15;5:1015–1021. doi: 10.1016/j.dib.2015.11.016

Fig. 2.

Fig. 2.

SeC-MC reduce muscle necrosis when i.p. injected into presymptomatic dystrophic mice. (A) Two-week-old mdx mice were i.p. injected with E-MC (mock) (n=6) or SeC-MC (n=6). Three weeks after injection TA muscle morphology was analyzed by haematoxylin/eosin staining (lower left panels) and the mean percentages (±SEM) of undamaged, centrally-nucleated, and necrotic myofibers were determined (lower right panels).TA muscles of untreated 2-week- and 5-week-old mdx mice (n=5 each group) were analyzed in parallel (upper panels). (B) TA muscles from mock- and SeC-MC-treated mdx mice in (A) were analyzed for infiltrating macrophages by MAC3 immunohistochemistry (brown). The mean percentages (±SEM) of MAC3+ areas were determined. (C) Utrophin expression was analyzed by Western blotting. The average relative densities (±SEM) of utrophin bands with respect to α-actinin bands were determined. (D) Utrophin localization was determined by immunofluorescence on formalin-fixed paraffin-embedded muscle tissue. Nuclei were counterstained with DAPI (blue). Shown are representative images. *, significantly different from control (p≤0.001). Original magnification (A, B, and D), 40×.