FIG. 2.

Potential off-target genomic sites for the NEUROG2 sgRNA. (A) In silico prediction of potential off-target sites by CasOT program. Generally, if the combined number of mismatches (Mm's) within seed and nonseed sequence is >4, off-target activities are unlikely. If the number of Mm's within the seed sequence is <2, off-target events need to be monitored and examined. A complete list of off-target prediction is shown in Supplementary Table S4. Two sites in exons and three sites in introns are shown here. (B) No mutation is detected in any locus in CRISPR/Cas9 clones as shown by sequencing. Color images available online at www.liebertpub.com/scd