FIG. 7.

The hygromycin resistance gene offers an additional selection method. The NEUROG2-mCherry-hygromycin resistance knockin reporter is designed for purification by either FACS or drug selection after differentiation. NEUROG2 reporter cells are differentiated toward neural lineage for 7 days, then treated with hygromycin. In 10 μg/mL hygromycin treated dishes (A–C), about 30% cells are mCherry+ as detected under fluorescence microscope, while in 50 μg/mL hygromycin treated dishes (D–F) and 66%–70% of total cell population is mCherry(+). Cell counting is done with ImageJ software. Quantification is done using Student's t-test (G). The experiments have been repeated in clones HRN2, CAN60, CAN82, and CAN127, for three times per group. **P < 0.001. Color images available online at www.liebertpub.com/scd