Table 1. Strains used in this study.
Strain Name | Strain Ref | Parental Strain | Key Genotype | Reference |
---|---|---|---|---|
SN152 | WT | SC5314 | arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434;IRO1/iro1::imm434 | (Noble and Johnson 2005) |
CaLY8 | SN152 | orf19.7494::HIS1/ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
PMET3-MMS22 | CaLY226 | CaLY8 | orf19.7494::HIS1/SAT1-MET3p-ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study |
CaLY219 | SN152 | ORF19.4136/orf19.4136::LEU2 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
PMET3-TOF1 | CaLY337 | CaLY219 | orf19.4136::LEU2/ARG-MET3p-ORF19.4136 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study |
CaLY220 | SN152 | ORF19.4105/orf19.4105::LEU2 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
PMET3-CSM3 | CaLY249 | CaLY220 | orf19.4105::LEU2/ARG4-MET3p-ORF19.4105 arg4/arg4; leu2/leu2; his1/his1; URA3/ura3::imm434;IRO1/iro1::imm434 | This study |
CaLY222 | SN152 | ORF19.658/orf19.658::LEU2 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
PMET3-MRC1 | CaLY316 | CaLY222 | orf19.658::LEU2/ARG4-MET3p-CaORF19.658 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study |
CaLY223 | SN152 | ORF19.2174/orf19.2174::LEU2 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
Δrad57 | CaLY235 | CaLY223 | orf19.2174::LEU2/orf19.2174::ARG4 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study |
CaLY224 | SN152 | ORF19.2440/orf19.2440::LEU2 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
Δrtt101 | CaLY236 | CaLY224 | orf19.2440::LEU2/orf19.2440::ARG4 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study |
CaLY228 | CaLY226 | ORF19.658/orf19.658::LEU2 orf19.7494::HIS1/SAT1-MET3p-ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
PMET3-MMS22/PMET3-MRC1 | CaLY251 | CaLY228 | orf19.658::LEU2/ARG4-MET3p-CaORF19.658 orf19.7494::HIS1/SAT1-MET3p-ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study |
CaLY234 | CaLY226 | ORF19.4105/orf19.4105::LEU2 orf19.7494::HIS1/SAT1-MET3p-ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
PMET3-MMS22/PMET3-CSM3 | CaLY246 | CaLY234 | orf19.4105::LEU2/ARG4-MET3p-ORF19.4105 orf19.7494::HIS1/SAT1-MET3p-ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study |
CaLY238 | CaLY226 | ORF19.2174/orf19.2174::LEU2 orf19.7494::HIS1/SAT1-MET3p-ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
PMET3-MMS22/Δrad57 | CaLY242 | CaLY238 | orf19.2174::LEU2/orf19.2174::ARG4 orf19.7494::HIS1/SAT1-MET3p-ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study |
CaLY240 | CaLY226 | ORF19.2440/orf19.2440::LEU2 orf19.7494::HIS1/SAT1-MET3p-ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study | |
PMET3-MMS22/Δrtt101 | CaLY244 | CaLY240 | orf19.2440::LEU2/orf19.2440::ARG4 orf19.7494::HIS1/SAT1-MET3p-ORF19.7494 arg4/arg4 leu2/leu2 his1/his1 URA3/ura3::imm434 IRO1/iro1::imm434 | This study |
WT, wild type.