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. 2015 Oct 2;5(12):2601–2610. doi: 10.1534/g3.115.020933

Figure 6.

Figure 6

Results of transposition efficiency analysis of silver carp Thm3 transposase in blunt snout bream. (A) The donor plasmid construct pThm1-Mlyz2-RFP harbors the left (367 bp) and right (230 bp) silver carp Thm1 inverted terminal repeats (ITRs). This also contains the zebrafish myosin light chain 2 (Mlyz2) promoter, the red fluorescent protein (RFP), and the SV40 poly(A) signal. (B) The transposase plasmid construct pCS2-CMV-Thm3TP. Capped silver carp Thm3 transposase mRNAs were synthesized in vitro, using linearized pCS2-CMV-Thm3TP plasmid DNA as a template. Light (C) and fluorescence (D) microscopy images of RFP expression in blunt snout bream embryos at 72 hpf after coinjection of the donor plasmid pThm1-Mlyz2-RFP and capped silver carp Thm3 transposase mRNAs in embryos at the one- to two-cell stage. Optical images of RFP expression in the negative control (E) and in transgenic positive individuals (F) in 200-d-old blunt snout bream. Arrows indicate RFP expression signals. White scale bars = 600 µm. Dark scale bars = 2 cm.