Figure 2.

Perithecial development in Δgpr-1, Δgpr-2, and Δgpr-3 mutants lacking CRL class G protein−coupled receptorr. Synthetic crossing medium cultures of the indicated strains containing protoperithecia were fertilized using opposite mating type conidia from a wild-type strain. Perithecia were photographed 10 d later. The arrows indicate normal perithecial beaks (wild type), perithecia with beaks that lack ostioles (Δgpr-1) or perithecia with beaks that bend downward or are torn during ascospore ejection (single, double or triple mutants lacking gpr-2 or gpr-3).