Table 1. Neurospora crassa G protein−coupled receptor gene families and summary of growth/developmental and chemical sensitivity/nutrition phenotypes.

Fungal GPCR Class	Description GPCR Class	NCUa	N. crassa Gene(s)b	Saccharomyces cerevisiae Homologc	Aspergillus nidulans Homologd	Linear Growthe	Asexual Developmentf	Sexual Developmentg	Chemical Sensitivityh/ Nutritioni
I	Fungal Pheromone	05758	pre-2	STE2	gprA		AH	PP, P, Aj
II	Fungal Pheromone	00138	pre-1	STE3	gprB			P, Aj
III	Carbon Sensory	06312	gpr-4	GPR1	None		AH
IV	Stm1-like	00300	gpr-5	YPQ1	AN5720				C
IV	Stm1-like	09195	gpr-6	RTC2	AN5720				C
V	cAMP Receptor-Like	00786	gpr-1	None	AN8262		AH	P	C
V	cAMP Receptor-like	04626	gpr-2	None	AN8262		AH	P
V	cAMP Receptor-like	09427	gpr-3	None	AN8262		AH	P
VI	GprK-like/RGS Domain	09883	gpr-7	None	AN7795 (gprK)				F
VII	Rat growth hormone releasing factor receptor-like	03253	gpr-8	None	AN6680	NAk
VIII	mPR-Like/PAQR	03238	gpr-9	IZH3	AN4932				S
VIII	mPR-like/PAQR	04987	gpr-10	IZH2	AN4932				F
IX	Microbial Opsin	10055	nop-1	YRO2, MRH1, HSP30	AN3361		AH		M
IX	Microbial Opsin	01735	orp-1	YRO2, MRH1, HSP30	AN3361		AH
X	Lung 7TM Superfamily	00182	gpr-11	PTM1	AN0063				YE
XI	GPCR89/ABA GPCR	00005	gpr-12	YHR078w	None
XII	Family C-like	06629	gpr-13	None	AN8601
XIII	DUF300 superfamily/Ps GPR11	06987	gpr-14	YKR051W	AN2232	NAk
XIVl	Pth11-like	00700	gpr-15	None	AN12202	R		PP, P
	Pth11-like	02903	gpr-16	None	AN0178		AH		F
	Pth11-like	04106	gpr-17	None	AN7774		AH
	Pth11-like	04931	gpr-18	None	AN10886	NAk
	Pth11-like	05101	gpr-19	None	AN1930	NAk
	Pth11-like	05187	gpr-20	None	AN8661	NAk
	Pth11-like	05189	gpr-21	None	AN8661
	Pth11-like	05307	gpr-22	None	AN0751		AH
	Pth11-like	05829	gpr-23	None	AN0751	R	AH		C,T
	Pth11-like	05854	gpr-24	None	AN8971				FL
	Pth11-like	06531	gpr-25	None	AN5639				C
	Pth11-like	17171	gpr-26	None	AN5664	NAk
	Pth11-like	07538	gpr-27	None	AN5664				F
	Pth11-like	16721	gpr-28	None	AN2249	NAk
	Pth11-like	07649	gpr-29	None	AN8943	R	AH		S,C
	Pth11-like	07769	gpr-30	None	AN4452		AH
	Pth11-like	08429	gpr-31	None	None	I	AH		F
	Pth11-like	08431	gpr-32	None	AN5664				A
	Pth11-like	08447	gpr-33	None	AN5664
	Pth11-like	08624	gpr-34	None	AN0178
	Pth11-like	08718	gpr-35	None	AN8951
	Pth11-Like	09022	gpr-36	None	AN5664				A
	Pth11-like	09201	gpr-37	None	AN5664
	Pth11-like	09796	gpr-38	None	AN8328	R
	Pth11-like	09823	gpr-39	None	AN1930		AH		A

GPCR, G protein−coupled receptor; NCU, N. crassa gene number.

^aBased on the Broad Institute’s Neurospora crassa database (http://www.broadinstitue.org/annotation/genome/neurospora/MultiHome.html).

^bN. crassa gene names are from the literature or designated during this study.

^cYeast orthologs were obtained after a Blastp search of the NCBI database, using a cut-off score of e-05 or less.

^dA. nidulans orthologs were obtained from a Blastp search of the NCBI database, using a cut-off score of e-05 or less. Only the top hit is shown.

^eR, reduced growth rate; I, increased growth rate relative to wild type.

^fDefects in aerial hyphae (AH) or conidial development (C).

^gDefects in protoperithecial (PP), perithecial (P), and/or ascospore (A) development.

^hChemical sensitivity phenotypes are indicated based on sensitivity or resistance to sorbitol (S), cytochalasin A (C), benomyl (B), tert-butyl hydroperoxide (T), menadione (M), FK506 (F), and fludioxonil (FL).

ⁱRepresented by increased or decreased growth on Avicel (A) or Yeast Extract (Y) compared with wild type.

^jMating type dependent.

^kNot available; see Table S1 for details.

^lCategory XIV named in this study.