Table 2. G protein−coupled receptor mutants with chemical sensitivity and nutritional phenotypes.

NCU	N. crassa Gene	Sorbitol	Peroxide	Menadione	FK506	Cytochalasin A	Fludioxonil	Avicel	Yeast Extract
00300	gpr-5					R (+29%)
09195	gpr-6					R (+25%)
00786	gpr-1					S (-13%)
09883	gpr-7				R (+31%)
03238	gpr-9	R (+11%)
04987	gpr-10				R (+15%)
10055	nop-1			R (+26%)
00182	gpr-11								I (+18%)
02903	gpr-16				R (+18%)
05829	gpr-23		S (-25%)			S (-29%)
05854	gpr-24						S (-29%)
06531	gpr-25					R (+25%)
07538	gpr-27				R (+19%)
07649	gpr-29	R (+21%)				S (-28%)
08429	gpr-31				R (+16%)
08431	gpr-32							I (+39%)
09022	gpr-36							I (+47%)
09823	gpr-39							I (+30%)

Mutants were considered significantly different than wild type if the results of a T-test yielded P ≤ 0.20 for all three replicates and P ≤ 0.05 for at least two of the three replicates. For chemical sensitivity assays, R = more resistant than wild type and S = more sensitive than wild type. For nutritional phenotypes (yeast extract and avicel), I = increased growth relative to wild type. The percent difference relative to wild type is shown in parentheses for affected mutants. NCU, N. crassa gene number.