Figure 1.

Schematic of cell-specific RNA-sequation. (A) Embryos harvested using strains that give rise to pure populations of either sex. (B) Animals were hatched in the absence of food to synchronize growth. After hatching, larvae were fed to allow development to proceed. (C) Animals were harvested at the appropriate developmental time point by disrupting the cuticle and dissociating and filtering the single cells. (D) The majority of the dissociated cell suspension was sorted by FACS to isolate the gonadal cells. An aliquot of the dissociated cells was removed prior to FACS to be used as a whole animal control. (E) RNA was isolated and RNA-seq performed (see Materials and Methods).