Table 2.
Substrate uptake of amino acids and glucose supplied in MDM
| Substrate | Initial concentration | Relative abundance in stationary phase | thalfmaximal comsumption | Maximal relative uptake rate |
|---|---|---|---|---|
| g/L | % | min | h−1 | |
| Glucose | 2.0 | bdla | 197 ± 10 | 0.17 ± 0.01 |
| Methionine | 0.2 | bdl | 136 ± 17 | 0.23 ± 0.04 |
| Cysteine | 2.0 | bdl | 164 ± 13 | 0.21 ± 0.01 |
| Isoleucine | 0.3 | 18.4 ± 0.8 | 306 ± 18 | 0.11 ± 0.01 |
| Tryptophan | 0.1 | bdl | 307 ± 23 | 0.13 ± 0.01 |
| Proline | 2.0 | bdl | 318 ± 4 | 0.56 ± 0.05 |
| Leucine | 0.4 | 27.2 ± 0.2 | 332 ± 8 | 0.16 ± 0.01 |
| Valine | 0.3 | 36.3 ± 0.9 | 347 ± 8 | 0.18 ± 0.02 |
Total sampling range up to 720 min, for detailed fits, see Additional file 2; abdl, below detection limit