Fig. 6.

Relative gene expression of DEGs for RNA-Seq data validation. Fold change between 3mlpa and 3MWT at respective seed developmental stages for genes encoding (a) Cellulose synthase (CESA: Glyma12g36570) and (b) Sugar transporter (SugT1: Glyma08g06420). Green and orange bars indicate mean fold change values from RNA-Seq and qPCR experiments, respectively. There was no significant difference in the gene expression profiles estimated using qPCR and RNA-Seq analyses at significance level of 0.01 (See Additional file 8 for more information)