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. 2015 Dec 18;13:92. doi: 10.1186/s12951-015-0152-y

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© Min et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Astrocytic GABA on MW-CNTs spreads into cell processes. a–c Immunostaining of GABA using anti-GABA (red), anti-GFAP (green) antibody in primary cortical astrocytes on MW-CNT and PDL coverslips. a shows distribution of astrocytic GABA near the nucleus. b shows astrocytes on MW-CNT-50 to have a rounder shape compared to those on PDL and c shows distinct cell–cell interactions on MW-CNT-1000. a–c shows that intracellular GABA on MW-CNT has spread into cell processes (b, c) compared to the PDL coverslip (a); Scale bar 20 μm. d Graph shows MW-CNTs increase GFAP immunoreactivity compared to PDL (*p < 0.05); PDL (n = 7), MW-CNT-50 (n = 7), MW-CNT-1000 (n = 7). Fluorescence intensity (i.u.) = cell intensity − (area × mean fluorescence of background)