Fig. 4.

Growth phenotype of wild type, and ΔcitC and Δnog1. a Growth curves of wild type, and ΔcitC and Δnog1 strains grown separately in Schott bottles under shaking at 37 °C. The average OD values and the standard deviations from three replicates are given. b Percentage of strains after growth in competitive growth assays. Gray columns indicate plain LB medium, green columns LB supplemented with MgCl₂. The experiments were repeated at least three times and standard deviations are shown. First column, growth of Δnog1 against wild type in LB; second column, Δnog1 against wild type in LB with MgCl₂; third column, growth of ΔcitC against wild type in LB; fourth column, growth of Δnog1 against ΔcitC in LB; fifth column, growth of Δnog1 against ΔcitC in LB with MgCl₂; sixth column, growth of Δnog1 + pBAD competing against wild type + pBAD in LB with MgCl₂; seventh column, Δnog1 + pBAD::nog1 was grown together with wild type + pBAD in LB with MgCl₂. The last two experiments were performed in the presence of ampicillin and arabinose