Fig. 6.

Expression of R. prowazekii strain Brienl candidate sRNAs during host cell infection. The R. prowazekii strain Brienl sRNA candidates #1,#5, #9, #10, #24, and #25 were tested for their expression during infection of HMEC by RT-PCR (n = 3). The band sizes shown on the left side correspond to the 100 bp DNA ladder (New England Biolabs). The lane 2 (-RT) is a “no reverse transcriptase” control, while the lane 3 (Crtl) is an uninfected HMEC control. Lanes 4 through 10 are the samples from R. prowazekii strain Brienl infected HMECs from 1.5h to 72h post infection. All the tested sRNA candidates showed expression during host cell infection