Table 4.
Spirulina-S1 dataset | Maize-Rye dataset | |||
---|---|---|---|---|
Initial # reads | 1019333 | 1019333 | ||
Initial # ORF | 6115998 | 6115998 | ||
Hits | % | Hits | % | |
COG | ||||
Total # hits | 206325 | 323947 | ||
Information storage and processing | 47949 | 23.2a | 85124 | 26.3a |
Cellular processes and signaling | 37543 | 18.2a | 59222 | 18.3a |
Metabolism | 89072 | 43.2a | 124053 | 38.3a |
Poorly characterized | 31761 | 15.4a | 55548 | 17.1a |
Ratio AaM/CMb | 1.27 | 0.91 | ||
PFAMs | ||||
Total identified PFAMs | 1132766 | 2205177 | ||
Cellulose degradation PFAMs | 1881 | 0.17c | 6554 | 0.30c |
Amino acid degradation PFAMs | 10628 | 0.94c | 16052 | 0.73c |
Protein degradation PFAMs | 7148 | 0.63c | 11498 | 0.52c |
Ratio proteases/cellulases (P/C) | 3.80 | 1.75 | ||
Ratio amino acid/cellulases (A/C) | 5.65 | 2.45 |
a% of total identified COGs
b“Amino acid transport and metabolism”/“Carbohydrate transport and Metabolism”
c% of total identified PFAMs
Comparison of the COG categories obtained with the MG-Rast platform (E-value 1e−5; min 60 % identity; 15 bp min length) and the selected Pfams obtained with the Hmmscan (E-value cutoff 1.0)