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. 2015 Dec 18;15:280. doi: 10.1186/s12866-015-0611-5

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© Willing et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Cell wall proteins and culture supernatants of the mfd mutant. Cultures were grown in BHIS broth and cell wall proteins and culture supernatants were prepared and analyzed by 12 % SDS-PAGE. A high molecular weight band (▶) was observed in the culture supernatant of the mfd mutant, which was identified as toxin A. The phase variable CwpV protein is observed in the parental Δerm culture (▷). CWP, cell wall proteins; SN, culture supernatants; M, molecular weight markers