Table 1.

PCR primers used to assay gene expression

Gene	Primer	Sequence	Accession number	Tm (°C)	Product size (bp)
BDNF	Forward	5′-TGG CTG ACA CTT TTG AGC AC-3′	NM_001048142	55	188
	Reverse	5′-GTT TGC GGC ATC CAG GTA AT-3′
Exon I	Forward	5′-TGA GAG TTG AAG CTT TGC GG-3′	EF125669.1	55	189
	Reverse	5′-ATT GTG GCT TTG CTG TCC TG-3′
Exon IIC	Forward	5′-TTT GGT CCC CTC ATT GAG CT-3′	EF125672.1	55	159
	Reverse	5′-TTC TTT GCG GCT TAC ACC AC-3′
Exon III	Forward	5′-TCT ATC ATC CCT CCC CGA GA-3′	EF125681.1	55	123
	Reverse	5′-AAC TGG GCT CAA GGA AGC AT-3′
Exon IV	Forward	5′-AGC ATG AAA TCT CCC AGC CT-3′	EF125673.1	55	213
	Reverse	5′-CGG TCC CCA AGG TTC TAG AC-3′
Exon V	Forward	5′-TAG CTT TGT GGT GCG GGA AG-3′	EF125682.1	57	146
	Reverse	5′-AAG TTG CCT TGT CCG TGG A-3′
Exon VI	Forward	5′-GGG CTT GGA GAA GGA AAC CG-3′	EF125674.1	55	188
	Reverse	5′-GGT CCA CAC AAA GCT CTC GG-3′
Exon VII	Forward	5′-CTG TCA CCT GCT CTC TAG GG-3′	EF125683.1	55	118
	Reverse	5′-AGT TCC GCA GAC CCT TTC AG-3′
Exon VIII	Forward	5′-CAA CTG GAT GTG TGG AAC CA-3′	EF125684.1	55	128
	Reverse	5′-AGT GTG TGG GTA GAT GCC AA-3′
Exon IXA	Forward	5′-ATT TGT GTC CCC TGC AGC T-3′	EF125685.1	55	151
	Reverse	5′-GTG GGA AGG AAG CAG AGA CA-3′
GAPDH	Forward	5′-TCC ATG ACA ACT TTG GCA TT-3′	NM_008084.2	55	377
	Reverse	5′-GTT GCT GTT GAA GTC GCA GG-3′

BDNF, brain-derived neurotrophic factor; GAPDH, glyceraldehyde three-phosphate dehydrogenase.