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. Author manuscript; available in PMC: 2015 Dec 18.
Published in final edited form as: Plant Cell Physiol. 2015 Aug 12;56(10):1981–1996. doi: 10.1093/pcp/pcv119

Fig. 8.

Fig. 8

Effect of cytochalasin D (CD) and DMSO (0.1%; control) on charasome growth and photosynthetic activity in Chara internodal cells. Cells were incubated in darkness for 2 weeks in order to degrade existing charasomes (data not shown) and subsequently exposed to light in media containing DMSO (control) or cytochalasin, respectively. (A) Maximum and minimum charasome area fractions after a 7 d treatment are compared in box-and-whisker plots. Shown are median values with upper and lower quartiles (boxes), whiskers indicating the 10th and 90th percentiles, and outliers (dots) with n≥16 for each plot. Charasome formation was significantly inhibited in cells treated with 10 μM CD (*P < 0.05, Kruskal–Wallis one-way analysis of variance on ranks). (B) Photosynthetic efficiency ΔF/Fm (ΔF/Fm′) of cells treated with various concentrations of CD and with 0.25% DMSO. Data are presented as mean values ± SD (n is the number of cells examined). Differences in photosynthetic activity between untreated and CD-treated cells were insignificant.