Figure 5. Deficiency in MMP14 activity inhibits bimodal fibril diameter distribution in tendons from adult mice.
Tail tendons from 7 week-old (A) WT, (B) Scx-Cre::Mmp14 lox/lox, and (C) Col-r/r mice. Larger diameter fibrils can be observed in the ECM of WT and Col-r/r postnatal tendons but only narrow diameter fibrils are observed in Mmp14-deficient postnatal tendons. Scale bars 500 nm.
Figure 5—figure supplement 1. Cleavage of the ¾-¼ collagen-I site is not required for release of fibrils in tendons from adult mice.
Electron microscopy images of tendons from 7-week-old (A) WT, (B) Scx-Cre::Mmp14 lox/lox, and (C) Col-r/r mice. Larger diameter fibrils occur in the ECM of WT and Col-r/r postnatal tendons but only narrow diameter fibrils occur in Mmp14-deficient postnatal tendons. Scale bars 2 µm.
Figure 5—figure supplement 2. Immuno-electron microscopy of Scx-Cre::Mmp14 lox/lox tendon.
(A) WT and (B) Scx-Cre::Mmp14 lox/lox tail tendon from P7 (7 days) mice were labeled with anti-MMP14 antibody. (C) Scx-Cre::Mmp14 lox/lox tendon labeled with anti-collagen-I antibody. Scale bars 500 nm.