Fig. 1.

Developmental expression of zebrafish pank2. WISH was performed from 1 to 48 hpf using a pank2-specific, DIG-labeled antisense probe of 625 bp. From the “cleavage period” to the somitogenesis the pank2 signal is diffuse (A, B, C). At 24 hpf the transcript is present in defined CNS regions (midbrain, hindbrain and midbrain–hindbrain boundary). At 48 hpf the signal in the brain region is still present and also appears in the main vessels and in vessels of the vascular plexus in the tail region (E). A, B, C dorsal view, D and E lateral view, anterior to the left. Cross-sections at the level of the head and trunk of 24 (F, G) and 48 hpf embryos (H). Abbreviations: m, midbrain; mhb, midbrain–hindbrain boundary; h, hindbrain; da, dorsal aorta: cv; cardinal vein; pcv, post cardinal vein; e, epidermis; el, ependymal layer. The figure shows representative images from at least three different experiments, with 20 embryos/stage.