Fig. 8.

pank2 knock-down is associated with increased cell death. AO staining of not injected- (B), ST-MO- (C) and P2-MO-injected (D) embryos (all displayed in full size in A) shows an increased number of positive (dying) cells (white arrowheads) in pank2 morphants, compared to controls. Other positive AO domains, such as eye (e) and olfactory pits (op), are expected at this developmental stage. All embryos are at 48 hpf, in lateral view, anterior to the left. Experiments were performed in duplicate, with at least 30 embryos per condition. The graph in E shows the AO cell counting in the forebrain region of 5 embryos from each condition.