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. Author manuscript; available in PMC: 2015 Dec 18.
Published in final edited form as: Nutr Cancer. 2015 Sep 18;67(7):1201–1207. doi: 10.1080/01635581.2015.1075051

Figure 4.

Figure 4

Representative autoradiographs of 32P-labeled oxidative DNA adducts resulting from rat liver microsomes/Cu(II)-mediated activation of E2 in the presence of vehicle (2% ethanol) or caraway extracts. Labeled adducts were resolved by two-directional PEI-cellulose TLC (D1 = 1 M formic acid/40 mM sodium phosphate, pH 6.0 onto a 6 cm Whatman no. 17 paper wick; D2 = isopropanol: 4 M ammonium hydroxide, 1:1) and detected by Packard InstantImager.