Skip to main content
. 2015 Dec 18;10(12):e0145385. doi: 10.1371/journal.pone.0145385

Table 1. Summary statistics of assembly and annotation for Nypa fruticans.

De novo assembly statistics
Total number of raw reads 24,607,427×2
Total number of clean reads 19,918,800×2
Total number of contigs 51,702
Unigenes (contigs after removing redundancy) 45,368
Mean length of unigenes (bp) 722
Median length of unigenes (bp) 460
N50 value of unigenes (bp) 1,096
Longest unigene (bp) 9,795
GC content 47.7%
Annotation statistics of unigenes
NR-blast 32,260 (71.11%)
InterProScan 24,650 (54.33%)
Blast2GO 18,761 (41.35%)
KEGG pathway 2,956 (6.52%)
SSR statistics
Di-nucleotide motifs 4,436 (55.74%)
Tri-nucleotide motifs 3,323 (41.76%)
Tetra-nucleotide motifs 174 (2.19%)
Penta-nucleotide motifs 10 (0.13%)
Hexa-nucleotide motifs 15 (0.19%)

The N50 value indicates that 50% of the entire assembly is contained in sequences equal to or larger than this value. NR: NCBI non-redundant protein database, InterProScan: a protein signature recognition tool, KEGG: Kyoto Encyclopedia of Genes and Genomes, SSR: simple sequence repeat.