Table 1. Summary statistics of assembly and annotation for Nypa fruticans.
De novo assembly statistics | |
Total number of raw reads | 24,607,427×2 |
Total number of clean reads | 19,918,800×2 |
Total number of contigs | 51,702 |
Unigenes (contigs after removing redundancy) | 45,368 |
Mean length of unigenes (bp) | 722 |
Median length of unigenes (bp) | 460 |
N50 value of unigenes (bp) | 1,096 |
Longest unigene (bp) | 9,795 |
GC content | 47.7% |
Annotation statistics of unigenes | |
NR-blast | 32,260 (71.11%) |
InterProScan | 24,650 (54.33%) |
Blast2GO | 18,761 (41.35%) |
KEGG pathway | 2,956 (6.52%) |
SSR statistics | |
Di-nucleotide motifs | 4,436 (55.74%) |
Tri-nucleotide motifs | 3,323 (41.76%) |
Tetra-nucleotide motifs | 174 (2.19%) |
Penta-nucleotide motifs | 10 (0.13%) |
Hexa-nucleotide motifs | 15 (0.19%) |
The N50 value indicates that 50% of the entire assembly is contained in sequences equal to or larger than this value. NR: NCBI non-redundant protein database, InterProScan: a protein signature recognition tool, KEGG: Kyoto Encyclopedia of Genes and Genomes, SSR: simple sequence repeat.