Fig 2. Generation of ouib mutant alleles by CRISPR/Cas9 system.

(A) A schema of the ouib gene showing the sgRNA target sites. Exons are shown as black boxes, the transcriptional start site as arrow, and sgRNA target sites as black triangles. (B) Sequences of sgRNA target sites. The 20 bp target sequence corresponding to each target site is indicated in orange, along with the neighboring NGG protospacer adjacent motif (PAM) in pink and the cleavage site of Cas9 is shown as black triangles. (C) Predicted protein structures of ouib alleles. Ouib²⁹ and Ouib⁷⁴ are composed of 29 and 74 amino acids, respectively.