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. 2015 Dec 10;10(12):e0144638. doi: 10.1371/journal.pone.0144638

Fig 1. Effect of the W501R substitution on the ability of HCV helicase to unwind DNA.

Fig 1

(A) Representative fluorescence traces observed in molecular beacon-based helicase assays [36] with indicated amounts of each protein. The decrease in fluorescence intensity (RFU—Relative Fluorescence Unit) indicates higher DNA unwinding activity of the wildtype enzymes than NS3h_3a (W501R). (B) Initial rates of unwinding after ATP addition in each reaction fit to the Michaelis-Menten equation. The reactions were prepared containing 25 mM MOPS, 1.25 mM MgCl2, 5 nM Cy5-substrate; 1mM ATP, 0.05 mM DTT, 0.005 mg/mL BSA, 0.001% Tween20, and enzymes NS3h_1b (con1), NS3h_3a (wt) and NS3h_3a (W501R) at indicated concentrations.