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. 2015 Dec 11;11(12):e1005732. doi: 10.1371/journal.pgen.1005732

Fig 2. CHX pretreatment affects A-site occupancies in a coherent concentration-dependent manner.

Fig 2

Columns correspond to a series of experiments by Gerashchenko [34] using a gradient of concentrations of CHX, starting from no CHX on the far left and increasing to 100 times the standard concentration on the far right. Each column plots the measured A-site occupancies of all 61 non-stop codons for that concentration on a log scale. The width of the line connecting each codon identity across concentrations is scaled by the codon’s net change from no CHX to 100x CHX. The ten codon identities with the largest net changes are labeled. Most notably, the codon identities with the highest enrichments in the experiment with no CHX undergo dramatic, concentration-dependent depletions over the course of the gradient.